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In Depth Analysis of Kinase Cross Screening Data to Identify CAMKK2 Inhibitory Scaffolds.

Molecules (Basel, Switzerland). 2020-01-13; 
O'Byrne SN,Scott JW,Pilotte JR,Santiago ADS,Langendorf CG,Oakhill JS,Eduful BJ,Couñago RM,Wells CI,Zuercher WJ,Willson TM,Drewry DH
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Catalog Antibody 02% [v/v] Brij-35) containing 200 µM CaMKKtide (Genscript), 100 µM CaCl2 , 1 µM CaM (Sigma-Aldrich, Castle Hill, NSW, Australia) 200 µM [γ-32P]-ATP (Perkin Elmer, Boston, MA, USA), 5 mM MgCl2 (Sigma-Aldrich, Castle Hill, NSW, Australia) and various concentrations of inhibitors (0–1 µM) in a standard 30 µl assay for 10 min at 30 C Get A Quote

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