Products/Services Used | Details | Operation |
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GenParts™ DNA Fragments> | … and DNA fragment synthesis were performed by GenScript service (https://www.funakoshi.co. jp/contents/687) according to the information on D. discoideum codon usage (http://dictybase. org) and MaLionG sequences (Arai et al., 2018). The synthesized DNA fragment coding … | Get A Quote |
Multicellular organisms contain various differentiated cells. Fate determination of these cells remains a fundamental issue. The cellular slime mold Dictyostelium discoideum is a useful model organism for studying differentiation; it proliferates as single cells in nutrient-rich conditions, which aggregate into a multicellular body upon starvation, subsequently differentiating into stalk cells or spores. The fates of these cells can be predicted in the vegetative phase: Cells expressing higher and lower levels of omt12 differentiate into stalk cells and spores, respectively. However, omt12 is merely a marker gene and changes in its expression do not influence the cell fate, and determinant factors remain unknow... More