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Intracellular ATP levels influence cell fates in Dictyostelium discoideum differentiation

Genes Cells. 2020; 
Haruka Hiraoka, Tadashi Nakano, Satoshi Kuwana, Masashi Fukuzawa, Yasuhiro Hirano, Masahiro Ueda, Tokuko Haraguchi, Yasushi Hiraoka
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GenParts™ DNA Fragments … and DNA fragment synthesis were performed by GenScript service (https://www.funakoshi.co. jp/contents/687) according to the information on D. discoideum codon usage (http://dictybase. org) and MaLionG sequences (Arai et al., 2018). The synthesized DNA fragment coding … Get A Quote

摘要

Multicellular organisms contain various differentiated cells. Fate determination of these cells remains a fundamental issue. The cellular slime mold Dictyostelium discoideum is a useful model organism for studying differentiation; it proliferates as single cells in nutrient-rich conditions, which aggregate into a multicellular body upon starvation, subsequently differentiating into stalk cells or spores. The fates of these cells can be predicted in the vegetative phase: Cells expressing higher and lower levels of omt12 differentiate into stalk cells and spores, respectively. However, omt12 is merely a marker gene and changes in its expression do not influence the cell fate, and determinant factors remain unknow... More

关键词

ATP, ATP sensor probe, ATeam, MaLion, TCA cycle, cell differentiation, glycolysis, live-cell imaging, metabolism, mitochondria