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Soluble collectin-12 mediates C3-independent docking of properdin that activates the alternative pathway of complement

Elife. 2020; 
Jie Zhang, Lihong Song, Dennis V Pedersen, Anna Li, John D Lambris, Gregers Rom Andersen, Tom Eirik Mollnes, Ying Jie Ma, Peter Garred
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Recombinant Antibody Expression … Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States; 6Department of … found to be closely associated with several disease states including infections … recombinant properdin DNA encoding human properdin was synthesized (GenScript) with the … Get A Quote

摘要

Properdin stabilizes the alternative C3 convertase (C3bBb), whereas its role as pattern-recognition molecule mediating complement activation is disputed for decades. Previously, we have found that soluble collectin-12 (sCL-12) synergizes complement alternative pathway (AP) activation. However, whether this observation is C3 dependent is unknown. By application of the C3-inhibitor Cp40, we found that properdin in normal human serum bound to solely in a C3b-dependent manner. Cp40 also prevented properdin binding when properdin-depleted serum reconstituted with purified properdin was applied, in analogy with the findings achieved by C3-depleted serum. However, when opsonized with sCL-12, properdin bound in a C3-i... More

关键词

alternative pathway, collectin-12, immunology, infectious disease, inflammation, microbiology, properdin, the complement system