至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

The stability of HIV-2 Vpx and Vpr proteins is regulated by the presence or absence of zinc-binding sites and poly-proline motifs with distinct roles

J Gen Virol. 2020; 
Kazunori Shimagaki, Ryoko Koga, Haruna Fujino, Ami Ahagon, Hiroshi Tateishi, Masami Otsuka, Yoshihiro Yamaguchi, Mikako Fujita
Products/Services Used Details Operation
GenParts™ DNA Fragments … Other pGL-AN mutants with P104A, P105A, P106A and P107A in vpx were also constructed … DNA fragment [EcoRI site (5756)/NsiI site] of pGL-AN with this chimeric region and an R83C mutation subcloned into pUC57 (pUC-GL-rR83C/PPM) was prepared (GenScript) … Get A Quote

摘要

The Vpx and Vpr proteins of human immunodeficiency virus type 2 (HIV-2) are important for virus replication. Although these proteins are homologous, Vpx is expressed at much higher levels than Vpr. Previous studies demonstrated that this difference results from the presence of an HHCC zinc-binding site in Vpx that is absent in Vpr. Vpx has another unique region, a poly-proline motif (PPM) of seven consecutive prolines at the C-terminus. Using PPM point mutants of Vpx, this study demonstrated that these seven consecutive prolines are critical for suppressing proteasome degradation of Vpx in the absence of Gag. Both the PPM and the zinc-binding site stabilize Vpx but do so via different mechanisms. PPM and zinc-b... More

关键词

HIV-2, Vpr, Vpx, poly-proline, protein expression, zinc