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GenParts™ DNA Fragments> | … Other pGL-AN mutants with P104A, P105A, P106A and P107A in vpx were also constructed … DNA fragment [EcoRI site (5756)/NsiI site] of pGL-AN with this chimeric region and an R83C mutation subcloned into pUC57 (pUC-GL-rR83C/PPM) was prepared (GenScript) … | Get A Quote |
The Vpx and Vpr proteins of human immunodeficiency virus type 2 (HIV-2) are important for virus replication. Although these proteins are homologous, Vpx is expressed at much higher levels than Vpr. Previous studies demonstrated that this difference results from the presence of an HHCC zinc-binding site in Vpx that is absent in Vpr. Vpx has another unique region, a poly-proline motif (PPM) of seven consecutive prolines at the C-terminus. Using PPM point mutants of Vpx, this study demonstrated that these seven consecutive prolines are critical for suppressing proteasome degradation of Vpx in the absence of Gag. Both the PPM and the zinc-binding site stabilize Vpx but do so via different mechanisms. PPM and zinc-b... More