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Transcriptional and subcellular characterization of interferon induced protein-35 (IFP35) in mandarin fish, Siniperca chuatsi

Dev Comp Immunol. 2020; 
Li Li, Shan Nan Chen, Nan Li, P Nie
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DNA Sequencing … The PCR product was ligated into pMD18-T vector and sequenced by a commercial company (Sangon Biotech) … Simply, protein samples were separated on 4–20% SurePAGE (Genscript) under 140 V for 90 min, before being transferred electrophoretically to a 0.22 μM PVDF … Get A Quote

摘要

Interferon (IFN)-stimulated genes (ISGs) exert multiple functions in immune system, and IFN-induced protein 35 (IFP35), which is a member of ISG, has been suggested to be involved in numerous cellular activities including the regulation of antiviral immunity in mammals. However, the role of IFP35 in fish innate immunity remains largely unknown. In the present study, we characterized the IFP35 gene in mandarin fish Siniperca chuatsi, which contains two conserved Nmi/IFP35 homology domains (NIDs) at C-terminus, but no leucine zipper motif, with its genomic DNA sequence consisting of eight exons and seven introns. High and constitutive mRNA level of IFP35 was observed in all examined tissues, with the highest leve... More

关键词

IFN, IFN induced Protein-35, IFP35, Mandarin fish, Promoter, Transcription regulation