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Functional Studies of Trichoplax adhaerens Voltage-Gated Calcium Channel Activity

Methods Mol Biol. 2021; 
Julia Gauberg, Adriano Senatore, Andreas Heyland
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Mammalian Expression … This is the most convenient concentration for transfections. 6. Trichoplax DNA can be codon-optimized for better expression in mammalian cells. This requires synthesis of the cDNA sequence by a company such as GenScript. 7 … Get A Quote

摘要

Trichoplax adhaerens is a member of the phylum Placozoa, an enigmatic group of benthic animals with remarkably simple morphology. While initial work on these organisms has primarily focused on their morphology and the development of genomic resources, Trichoplax has received increased attention as a model for studying the evolution of nervous and sensory systems. This work is motivated by the fact that Trichoplax features distinct behaviours and responses to environmental stimuli. Therefore, much progress has been made in recent years on the molecular, cellular, and behavioral understanding of this organism. Methods outlined here provide hands-on approaches to cutting edge molecular and cellular techniques to r... More

关键词

Calcium channels, Cloning, Patch clamp, Placozoa