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Optimized Expression and Characterization of a Novel Fully Human Bispecific Single-Chain Diabody Targeting Vascular Endothelial Growth Factor165 and Programmed Death-1 in and Evaluation of Antitumor Activity In Vivo

Int J Mol Sci. 2018; 
Chenghao Xiong, Yingqing Mao, Tao Wu, Nannan Kang, Mingjun Zhao, Rongrong Di, Xiaoping Li, Xuemei Ji, Yu Liu
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Recombinant Antibody Expression … The resulting sequence was synthesized by Genscript (ID#: C4608CE150-1) and subcloned into the pMac-L vector … Example 6. Recombinant Antibodies Induced Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) … Get A Quote

摘要

Bispecific antibodies, which can bind to two different epitopes on the same or different antigens simultaneously, have recently emerged as attractive candidates for study in various diseases. Our present study successfully constructs and expresses a fully human, bispecific, single-chain diabody (BsDb) that can bind to vascular endothelial growth factor 165 (VEGF165) and programmed death-1 (PD-1) in . Under the optimal expression conditions (methanol concentration, 1%; pH, 4.0; inoculum density, OD600 = 4, and the induction time, 96 h), the maximum production level of this BsDb is achieved at approximately 20 mg/L. The recombinant BsDb is purified in one step using nickel-nitrilotriacetic acid (Ni-NTA) column ch... More

关键词

Pichia pastoris, anti-angiogenesis, bispecific single-chain diabody, immunotherapy, programmed death-1, vascular endothelial growth factor165