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Inhibition of Sonic Hedgehog Signaling Suppresses Glioma Stem-Like Cells Likely Through Inducing Autophagic Cell Death

Front Oncol. 2020; 
Hui-Chi Hung, Chan-Chuan Liu, Jian-Ying Chuang, Chun-Lin Su, Po-Wu Gean
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ORF cDNA Clones/MolecularCloud … To create lentiviruses over-expressing Shh, full-length Shh open reading frames (ORFs) (NM_000193; GenScript, New Jersey, USA) was amplified by … Single treatment of CD133 + cells with LDE225 (25 μM) or rapamycin (100 nM) inhibited the cell viability by 32.0 ± 7.9% (n = 6 … Get A Quote

摘要

Glioblastoma (GBM) often recurs after radio- and chemotherapies leading to poor prognosis. Glioma stem-like cells (GSCs) contribute to drug resistance and recurrence. Thus, understanding cellular mechanism underlying the growth of GSCs is critical for the treatment of GBM. Here GSCs were isolated from human U87 GBM cells with magnetic-activated cell sorting (MACS) using CD133 as a marker. The CD133 cells highly expressed sonic hedgehog (Shh) and were capable of forming tumor spheroids and tumor . Athymic mice received intracranial injection of luciferase transduced parental and CD133 GBM cells was utilized as orthotopic GBM model. Inhibited Shh by LDE225 delayed GBM growth , and downregulated Ptch1 and Gli1. C... More

关键词

amiodarone, autophagy, glioblastoma, sonic hedgehog, stem-like cells