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An efficient virus-induced gene silencing (VIGS) system for functional genomics in Brassicas using a cabbage leaf curl virus (CaLCuV)-based vector

Planta. 2020; 
Zhiliang Xiao, Miaomiao Xing, Xing Liu, Zhiyuan Fang, Limei Yang, Yangyong Zhang, Yong Wang, Mu Zhuang, Honghao Lv
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GenParts™ DNA Fragments … The palindromic DNA fragment was produced by the Nanjing Genscript Technology Company in Page 7. 7 … Page 10. 10 confirm whether the gene expressions of target genes were knocked down. The gene function of … Get A Quote

摘要

CaLCuV-based VIGS effectively works in cabbage and contributes to efficient functional genomics research in Brassica crop species. Virus-induced gene silencing (VIGS), a posttranscriptional gene silencing method, is an effective technique for analysing the functions of genes in plants. However, no VIGS vectors have been available for Brassica oleracea until now. Here, tobacco rattle virus (TRV), pTYs and cabbage leaf curl virus (CaLCuV) gene-silencing vectors (PCVA/PCVB) were chosen to improve the VIGS system in cabbage using the phytoene desaturase (PDS) gene as an efficient visual indicator of VIGS. We successfully silenced the expression of PDS and observed photobleaching phenomena in cabbage in response to ... More

关键词

Brassicas, CaLCuV, Cabbage, Phytoene desaturase (PDS), VIGS system, pTYs