Products/Services Used | Details | Operation |
---|---|---|
ORF cDNA Clones/MolecularCloud> | … The dialysis membrane (MWCO 3.5 kDa) was purchased from Spectrum Laboratories, Inc … Both 4 μg of DNA (GPR119 cDNA ORF clone, Homo sapiens; OHu18794, GenScript) and 0.65 μg of branched-polyethylenimine (bPEI, MW; 25 kDa) were respectively diluted in 250 μL of … | Get A Quote |
"K-cell targeted photodynamic therapy (K-cell targeted PDT)" with a minimally invasive procedure was investigated to reduce the secretion of gastric inhibitory polypeptide (GIP), an incretin hormone secreted from enteroendocrine K-cells in the duodenum that is suspected to be strongly correlated with obesity. Oleic acid-poly (ethylene glycol)-chlorin e6 (OA-PEG-Ce6, OPC) was designed for PDT because it targets K-cells through the interaction of OA in OPC with a G protein-coupled receptor (GPR 119) that is overexpressed on K-cells and mediates fatty acid sensing. OPC interacted with duodenal cells (HUTU-80) expressing GPR 119 and HEK 293 cells transfected with human GPR 119 to mimic K-cells in vitro. The intrac... More