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Gas Permeability of Mold during Freezing Process Alters the Pore Distribution of Gelatin Sponge and Its Bone-Forming Ability

Materials (Basel). 2020; 
Xiaoyu Han, Yoshitomo Honda, Tomonari Tanaka, Kazuki Imura, Yoshiya Hashimoto, Kazushi Yoshikawa, Kazuyo Yamamoto
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Endotoxin Detection & Removal System … The LPS contents of the sponge solutions were assessed using the ToxinSensor Chromogenic LAL Endotoxin Assay Kit (GenScript Biotech Corporation, Piscataway, NJ, USA) according to the manufacturer's instructions. 2.6. Cytotoxicity of the Sponges … Get A Quote

摘要

Freeze-drying, also known as lyophilization, is widely used in the preparation of porous biomaterials. Nevertheless, limited information is known regarding the effect of gas permeability on molds to obtain porous materials. We demonstrated that the different levels of gas permeability of molds remarkably altered the pore distribution of prepared gelatin sponges and distinct bone formation at critical-sized bone defects of the rat calvaria. Three types of molds were prepared: silicon tube (ST), which has high gas permeability; ST covered with polyvinylidene chloride (PVDC) film, which has low gas permeability, at the lateral side (STPL); and ST covered with PVDC at both the lateral and bottom sides (STPLB). The ... More

关键词

bone formation, freeze-dry, gas permeability, gelatin, lyophilization, pore distribution