至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

The intracellular lipid-binding domain of human Na/H exchanger 1 forms a lipid-protein co-structure essential for activity

Commun Biol. 2020-12; 
Ruth Hendus-Altenburger, Jens Vogensen, Emilie Skotte Pedersen, Alessandra Luchini, Raul Araya-Secchi, Anne H Bendsoe, Nanditha Shyam Prasad, Andreas Prestel, Marité Cardenas, Elena Pedraz-Cuesta, Lise Arleth, Stine F Pedersen, Birthe B Kragelund
Products/Services Used Details Operation
PCR Cloning and Subcloning … necessary to support glycolytic flux. Materials and methods. Cloning, expression and purification. A truncated pyruvate kinase enzyme was generated using a nucleotide sequence synthesised by Genscript (Korea). The PK TIM+Reg … Get A Quote

摘要

Dynamic interactions of proteins with lipid membranes are essential regulatory events in biology, but remain rudimentarily understood and particularly overlooked in membrane proteins. The ubiquitously expressed membrane protein Na/H-exchanger 1 (NHE1) regulates intracellular pH (pH) with dysregulation linked to e.g. cancer and cardiovascular diseases. NHE1 has a long, regulatory cytosolic domain carrying a membrane-proximal region described as a lipid-interacting domain (LID), yet, the LID structure and underlying molecular mechanisms are unknown. Here we decompose these, combining structural and biophysical methods, molecular dynamics simulations, cellular biotinylation- and immunofluorescence analysis and exc... More

关键词