Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | … necessary to support glycolytic flux. Materials and methods. Cloning, expression and purification. A truncated pyruvate kinase enzyme was generated using a nucleotide sequence synthesised by Genscript (Korea). The PK TIM+Reg … | Get A Quote |
Dynamic interactions of proteins with lipid membranes are essential regulatory events in biology, but remain rudimentarily understood and particularly overlooked in membrane proteins. The ubiquitously expressed membrane protein Na/H-exchanger 1 (NHE1) regulates intracellular pH (pH) with dysregulation linked to e.g. cancer and cardiovascular diseases. NHE1 has a long, regulatory cytosolic domain carrying a membrane-proximal region described as a lipid-interacting domain (LID), yet, the LID structure and underlying molecular mechanisms are unknown. Here we decompose these, combining structural and biophysical methods, molecular dynamics simulations, cellular biotinylation- and immunofluorescence analysis and exc... More