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Titration of myelin oligodendrocyte glycoprotein (MOG) - Induced experimental autoimmune encephalomyelitis (EAE) model

J Neurosci Methods. 2020-11; 
Rina Aharoni, Renana Globerman, Raya Eilam, Ori Brenner, Ruth Arnon
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Custom DNA/RNA Oligos … 2.2. EAE induction and evaluation. The MEVGWYRSPFSRVVHLYRNGK peptide encompassing amino acids 35–55 of the myelin oligodendrocyte glycoprotein (MOG), synthesized by Genscript (Piscataway, NJ), was used. An … Get A Quote

摘要

background: Experimental autoimmune encephalomyelitis (EAE) induced by the myelin oligodendrocyte glycoprotein (MOG) peptide 35-55, is a widely used multiple sclerosis (MS) model. Unlike the spontaneous occurrence of MS, in EAE, external immunization with the MOG peptide (200-300 µg/mouse), emulsified in adjuvant enriched with Mycobacterium Tuberculosis (MT) H37Ra (100-500 µg mouse), and pertussis toxin (PTx, 200-500 ng/mouse) injections, are applied, which heavily boosts the immune system. unassigned: A detailed and systematic titration of the MOG 35-55 EAE induction protocol in C57BL/6 mice reveals the minimal doses of the MOG 35-55 peptide, MT H37Ra, and PTx, required for disease manifestation. resul... More

关键词

Experimental autoimmune encephalomyelitis, Improved titrated induction protocol, Myelin oligodendrocyte glycoprotein (MOG)