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E coli production process yields stable dengue 1 virus-sized particles (VSPs)

Vaccine. 2020; 
Janet Hirsch, Bart W Faber, James E Crowe, Babs Verstrepen, Gesine Cornelissen
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Bacterial Expression … The gene-sequence was codon-optimised for E. coli expression (GenScript, New Jersey, USA) and subsequently synthesized, cloned into the PJ412Express Expression vector (DNA2.0, Menlo Park, CA) and transformed to E. coli DE3 cells … Get A Quote

摘要

Dengue fever is one of the most wide-spread vector-borne diseases in the world. Although dengue-associated mortality is low, morbidity and economic impact are high. Current licensed vaccines are limited and mediate only partial protection, thus a cost-effective vaccine with improved efficacy is strongly needed. In this work, recombinant dengue serotype 1 E protein was produced in E. coli, inclusion bodies were isolated and the E protein solubilized in urea and purified using an immobilized metal chelate affinity column. The protein was refolded by dialysis in order to obtain virus-like particles (VLPs). Particle assembly was confirmed using size-exclusion chromatography, dynamic light scattering (DLS), transmis... More

关键词

Dengue, Dynamic light scattering, E. coli, Stability, Virus-like particle, Virus-sized particle