至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Ultrahigh Adhesion Force Between Silica-Binding Peptide SB7 and Glass Substrate Studied by Single-Molecule Force Spectroscopy and Molecular Dynamic Simulation

Front Chem. 2020-11; 
Xiaoxu Zhang, Jialin Chen, Enci Li, Chunguang Hu, Shi-Zhong Luo, Chengzhi He
Products/Services Used Details Operation
Peptide Synthesis … NHS) was purchased from Yarebio. The SB7 (RQSSRGRGGGC) and Car9 (DSARGFKKPGKRGGGC) peptides were synthesized by GenScript, Nanjing. The Arg7 (RRRRRRR) peptide was purchased from Allpeptide, Hanzhou … Get A Quote

摘要

Many proteins and peptides have been identified to effectively and specifically bind on certain surfaces such as silica, polystyrene and titanium dioxide. It is of great interest, in many areas such as enzyme immobilization, surface functionalization and nanotechnology, to understand how these proteins/peptides bind to solid surfaces. Here we use single-molecule force spectroscopy (SMFS) based on atomic force microscopy to directly measure the adhesion force between a silica-binding peptide SB7 and glass surface at single molecule level. SMFS results show that the adhesion force of a single SB7 detaching from the glass surface distributes in two populations at ~220 pN and 610 pN, which is higher than the unfold... More

关键词

adhesion, atomic force micorscopy (AFM), molecular dynamics simulation (MD), peptide, silica, single molecule force spectroscopy (SMFS)