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Identification of pathogenicity-related genes in the rice pathogen Ustilaginoidea virens through random insertional mutagenesis

Fungal Genet Biol. 2015; 
Mina Yu, Junjie Yu, Jiankun Hu, Lei Huang, Yahui Wang, Xiaole Yin, Yafeng Nie, Xiangkun Meng, Weiduo Wang, Yongfeng Liu
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Molecular Biology Tools … The primer pairs used for the subsequent qRT-PCR assays were designed using the GenScript real-time PCR primer design tool (www.genscript.com/tools/real … of the two parents to U. virens infection, qRT-PCR assay of PR genes was carried out in resistant line Nanjing and the … Get A Quote

摘要

Agrobacterium tumefaciens-mediated transformation (ATMT) is becoming a popular effective system as an insertional mutagenesis tool in filamentous fungi. To gain more insight into the cellular and molecular mechanisms in the pathogenesis of Ustilaginoidea virens, the causal agent of rice false smut disease, a T-DNA insertion mutant library of U. virens was established using ATMT. We optimized a range of conditions to improve the transformation efficiency. Transformants were most effectively obtained when the optimal co-cultivation time is 72h, with 50μM AS in medium and 100μl A. tumefaciens for co-cultivation, leading to the production of 160-185 hygromycin B resistant transformants per 1×10(5) conidia. South... More

关键词

Agrobacterium tumefaciens-mediated transformation, Rice false smut, T-DNA flanking sequences, Ustilaginoidea virens, Virulence gene