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Development and characterization of recombinant tick-borne encephalitis virus expressing mCherry reporter protein: A new tool for high-throughput screening of antiviral compounds, and neutralizing antibody assays

Antiviral Res. 2020-11; 
Jan Haviernik, Ludek Eyer, Kentaro Yoshii, Shintaro Kobayashi, Jiri Cerny, Antoine Nougairède, Jean-Sélim Driouich, Jiri Volf, Martin Palus, Xavier de Lamballerie, Ernest A Gould, Daniel Ruzek
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GenParts™ DNA Fragments … Three de novo synthesized DNA fragments cloned into a pUC57 or a PC11 vector were used in this study (GenScript, Piscataway, NJ, USA): fragment I (nucleotide position 1 to 3662), fragment II (nucleotide position 3545 to 8043), and fragment III (nucleotide position 7961 to … Get A Quote

摘要

The flavivirus, tick-borne encephalitis virus (TBEV) is transmitted by Ixodes spp. ticks and may cause severe and potentially lethal neurological tick-borne encephalitis (TBE) in humans. Studying TBEV requires the use of secondary methodologies to detect the virus in infected cells. To overcome this problem, we rationally designed and constructed a recombinant reporter TBEV that stably expressed the mCherry reporter protein. The resulting TBEV reporter virus (named mCherry-TBEV) and wild-type parental TBEV exhibited similar growth kinetics in cultured cells; however, the mCherry-TBEV virus produced smaller plaques. The magnitude of mCherry expression correlated well with progeny virus production but remained st... More

关键词

Antivirals, Neutralization test, Reporter virus, Tick-borne encephalitis virus