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MicroRNA-21-5p targets PDCD4 to modulate apoptosis and inflammatory response to Clostridium perfringens beta2 toxin infection in IPEC-J2 cells

Dev Comp Immunol. 2020; 
Xiaoli Gao, Xiaoyu Huang, Qiaoli Yang, Shengwei Zhang, Zunqiang Yan, Ruirui Luo, Pengfei Wang, Wei Wang, Kaihui Xie, Shuangbao Gun
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Recombinant Antibody Expression … and human embryonic kidney cell line (HEK293T) were obtained from the Beijing Beina Chuanglian Institute of Biotechnology (Beijing, China) … Then, the recombinant CPB2 protein was purified using High-Affinity Ni-Charged Resin FF (GenScript, New Jersey, USA), dialysised … Get A Quote

摘要

Clostridium perfringens (C. perfringens), a toxin-producing enteric pathogen, causes a variety of intestinal infections in humans and animals. C. perfringens beta2 (CPB2) toxin has been considered to be a strong virulence factor for C. perfringens infectious enteric diseases (CPED). Altered levels and functions of microRNA-21-5p (miR-21-5p) have been associated with apoptosis and inflammation response in pathological processes. However, little is known about its functional mechanism in CPED. Here, we found that miR-21-5p expressed in multiple tissues of pig, had a highest level in jejunum, and significantly upregulated in intestinal porcine epithelial cells (IPEC-J2) exposed to CPB2 toxin. Noteworthily, transfe... More

关键词

Apoptosis, Clostridium perfringens beta2 (CPB2) toxin, IPEC-J2 cell, Inflammation response, PDCD4, miR-21-5p