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Removing light chain-missing byproducts and aggregates by Capto MMC ImpRes mixed-mode chromatography during the purification of two WuXiBody-based bispecific antibodies

Protein Expr Purif. 2020;

Yan Wan, Ting Zhang, Yumeng Wang, Ying Wang, Yifeng Li

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Molecular Biology Reagents	… Louis, MO, USA). Precast SurePAGE 4–12% gradient Bis-Tris gels were purchased from Genscript (Nanjing, China), 20X MES running buffer, 4X LDS sample buffer and PageRuler Unstained Protein Ladder were purchased from Thermo Fisher Scientific (Waltham, MA, USA) …	Get A Quote

摘要

WuXiBody is a novel bispecific antibody (bsAb) platform developed by WuXi Biologics. Its key feature is the replacement of one parental antibody's CH1/CL constant region with the T cell receptor (TCR) constant domain, a design aimed at promoting cognate heavy chain (HC)-light chain (LC) pairing. BsAbs based on WuXiBody can adopt either asymmetric or symmetric format. LC-missing species, a byproduct frequently associated with bsAb containing sequence engineered Fab arm, is also identified during WuXiBody production. Nevertheless, WuXiBody's unique design greatly facilitates removal of this type of impurity, which can otherwise be difficult to clear. In this work, with two concrete cases (WuXiBodies with asymmetr... More