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Carbapenemase OXA-423: A Novel OXA-23 Variant in

Infect Drug Resist. 2020-11; 
Zhenghai Yang, Peng Wang, Ping Song, Xiaoning Li
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DNA Sequencing … The complete bla OXA-23 -like was sequenced using an ABI 3730 DNA Analyzer at GenScript Inc. (Nanjing, China). The nucleotide sequences of bla OXA-23 -like and deduced amino acid sequences were analyzed using BioEdit™ software and BLAST programs. Table 1 … Get A Quote

摘要

unassigned: A novel variant of OXA-23, named OXA-423, was identified in an clinical isolate. The aim of this study was to analyse the resistance phenotype of OXA-423. unassigned: The strain WY-0713 was isolated from an intensive care unit patient. PCR was used to detect the -like genes. Amplifying, cloning and sequencing were performed for the complete -like. The novel and its ancestor were cloned into the expression vector pET-28b(+), and transformed into Rosetta (DE3) for antibiotic susceptibility testing. SDS-PAGE, modified Hodge test and CarbaNP test were used for detecting the expression of OXA-423 and OXA-23. unassigned: PCR screening of WY-0713 was positive for -like genes. Sequencing of the PCR pr... More

关键词

Acinetobacter baumannii, CHDL, OXA-23, OXA-423, carbapenemase