| Products/Services Used | Details | Operation |
|---|---|---|
| Molecular Biology Reagents | … C for 30 s, at 45°C for 30 s, and extension at 72°C for 1 min, with a final extension of 7 min at 72°C. The amplified products were analyzed by electrophoresis on 3% agarose gel and were then sent to the corporation Genscript (Nanjing, Jiangsu, China) for Sanger sequencing … | Get A Quote |
objective: The vitamin K epoxide reductase complex subunit 1 (VKORC1) plays an important role in bone development and bone metabolism by influencing the vitamin K cycle. The aim of this study was to investigate the association between VKORC1 gene polymorphisms and bone mineral density and the risk of osteoporosis. methods: We determined VKORC1 gene polymorphisms (rs9923231 and rs9934488) by using polymerase chain reaction-restriction and Sanger sequencing method in 606 postmenopausal women including 288 osteoporosis patients and 318 healthy controls. results: No significant differences were observed in the rs9934488 polymorphisms between the osteoporosis group and controls. Subgroup analyses also indicated no p... More
