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Production of polyhydroxyalkanoate copolymers containing 4-hydroxybutyrate in engineered Bacillus megaterium

Int J Biol Macromol. 2020-12; 
Andrew J Cal, Rena E Kibblewhite, W Dirk Sikkema, Lennard F Torres, William M Hart-Cooper, William J Orts, Charles C Lee
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ORF cDNA Clones/MolecularCloud … CoA transferase (orfZ), succinate semialdehyde dehydrogenase (sucD), and 4-hydroxybutyrate dehydrogenase (4hbD) gene cluster from the Clostridium kluyveri DSM 555 genome sequence (NCBI CP000673) was synthesized by GenScript (Piscataway, NJ, USA) … Get A Quote

摘要

Despite being used as a common platform for the commercial production of many biochemicals, Bacilli are often overlooked as a source of industrial polyhydroxyalkanoates (PHAs), biodegradable plastic replacements. In addition to having a robust expression system, the lack of lipopolysaccharides and ease of lysis make Bacilli an attractive host for the production of PHAs. In this work, a Bacillus megaterium strain was engineered to generate poly(3-hydroxybutyrate-co-4-hydroxybutryate) (P[3HB-co-4HB]) copolymers, which are among the most useful and industrially-relevant copolymers. These copolymers had lower modulus and increased toughness, thus making the copolymer suitable for a broader range of applications. Du... More

关键词

4-hydroxybutyrate, Bacillus megaterium, Biodegradable plastic, Polyhydroxyalkanoate copolymers