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GenParts™ DNA Fragments> | … After amplification, the gel-purified VHH fragments were obtained and inserted into the phagemid vector provided by Genscript®. The vector was shown in Fig. S1 in the supplementary. Electrotransformation was performed for E. coli SS320(NB-biolab, China) and the bacteria … | Get A Quote |
Since EGFR is an important and effective target for tumor therapy in the clinic. Several monoclonal antibodies and nanobodies were proved to target domain III of EGFR. Regarding the increased attention on nanobodies, the present study aimed to generate nanobodies specifically against domain III. After camel immunization, a gene repertoire of sdAb fragments with a diversity of 3×10 clones was produced. Following the construction of two sdAb phage display libraries, the successful epitope binning was carried out to identify the nanobody with the designated epitope. Modelling of the identified nanobody and molecular docking studies illustrated the paratope and epitope. Docking analysis revealed that the paratope ... More