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DNA Sequencing> | … Bioscience Clontech, USA). Primers for RACE are listed in Supplementary Table 2. The amplified PCR product was purified and cloned into pMD19-T (TaKaRa, Japan) and sequenced (GenScript, Nanjing, China). After assembly, the … | Get A Quote |
Calcineurin B-like protein-interacting protein kinases () play essential roles in plant abiotic stress response. In order to better understand salt tolerance, we cloned and analyzed the gene from the halophyte . Phylogenetic analysis shows that belongs to a sister clade with the gene and is thought to localize to the plasma membrane. shows the highest expression level in the root under normal growth conditions, whereas after NaCl treatment, the highest expression was found in the blade. -overexpressing Arabidopsis plants have a higher seed germination rate, longer root length, and displayed higher salt tolerance than wild type seedlings under salt stress conditions. Furthermore, overexpression might enhan... More