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Genome-wide identification and expression profiling of the carotenoid cleavage dioxygenase (CCD) gene family in Brassica napus L

PLoS ONE. 2020; 
Xin-Tong Zhou, Le-Dong Jia, Mou-Zheng Duan, Xue Chen, Cai-Lin Qiao, Jin-Qi Ma, Chao Zhang, Fu-Yu Jing, Sheng-Sen Zhang, Bo Yang, Li-Yuan Zhang, Jia-Na Li
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Molecular Biology Tools … Predicted subcellular localization of BnCCD proteins and promoter analysis of BnCCD genes. The subcellular localization of BnCCD proteins were predicted using WoLF PSORT (http://www.genscript.com/tools/wolf-psort) [56] … Get A Quote

摘要

Carotenoid cleavage dioxygenase (CCD), a key enzyme in carotenoid metabolism, cleaves carotenoids to form apo-carotenoids, which play a major role in plant growth and stress responses. CCD genes had not previously been systematically characterized in Brassica napus (rapeseed), an important oil crop worldwide. In this study, we identified 30 BnCCD genes and classified them into nine subgroups based on a phylogenetic analysis. We identified the chromosomal locations, gene structures, and cis-promoter elements of each of these genes and performed a selection pressure analysis to identify residues under selection. Furthermore, we determined the subcellular localization, physicochemical properties, and conserved pro... More

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