| Products/Services Used | Details | Operation |
|---|---|---|
| Molecular Biology Reagents | … Western blot. The lung tissues or MLE-12 cells were lysed in lysis buffer (Cell Signaling, Boston, MA, USA) and cell debris was removed the supernatant was boiled for 5 min in loading buffer (GenScript, Nanjing, China) for 20 min … | Get A Quote |
Asthma is a chronic respiratory disease with high heterogeneity in human. Different mouse models have been applied for investigation of pathogenesis and treatment of asthma, which target on different cells, receptors and pathways. Interleukin (IL-) 28B, a member of λ-interferons, have been shown to play a protective role in OVA-induced asthma, which is antigen-specific and adaptive immune system dominant. However, the roles of IL-28B in protease-induced asthma, an adaptive immune system independent asthma, are still unclear. Here, we used plant-derived cysteine protease, papain to induce asthma in mice and found that IL-28B was capable of alleviating papain-induced asthma. Papain challenge lead to activation o... More
