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Combination of Copper Ions and Nucleotide Generates Aggregates from Prion Protein Fragments in the N-Terminal Domain

Protein Pept Lett. 2020; 
Noriyuki Shiraishi, Yoshiaki Hirano
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GenParts™ DNA Fragments … was performed as described previously [51, 53]. N- acetylated and C-amidated PrP fragments, Octa1, Octa2, Octa3, Octa4, PrP84−114, and PrP76−114, were purchased from GenScript. The concentration of PrP23-98 and PrP … Get A Quote

摘要

background: It has been previously found that PrP, which contains four highly conserved octarepeats (residues 60-91) and one partial repeat (residues 92-96), polymerizes into amyloid-like and proteinase K-resistant spherical aggregates in the presence of NADPH plus copper ions. objective: We aimed to determine the requirements for the formation of these aggregates. methods: In this study, we performed an aggregation experiment using N-acetylated and Camidated PrP fragments of the N-terminal domain, Octa1, Octa2, Octa3, Octa4, PrP, and PrP, in the presence of NADPH with copper ions, and focused on the effect of the number of copper-binding sites on aggregation. results: Among these PrP fragments, Octa4, containi... More

关键词

Octa4, PrP fragment, adenine nucleotide, aggregation, copper ion, pyridine nucleotide