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Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications

Engineering (Beijing). 2020; 
Zeyu Sun, Keyi Ren, Xing Zhang, Jinghua Chen, Zhengyi Jiang, Jing Jiang, Feiyang Ji, Xiaoxi Ouyang, Lanjuan Li
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Insect Expression … characteristics of HCoV-19 which is profoundly different to other coronavirus. In this 105 … model was made to highlight important glycan features related to COVID-19 109 pathogenesis … was synthesized (Genscript) with codons optimized for insect cell expression. Its 115 … Get A Quote

摘要

The COVID-19 pandemic has led to worldwide efforts to understand the biological traits of the newly identified HCoV-19 virus. In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 S protein, 20 are completely occupied by -glycans, predominantly of the oligomannose type. All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex -glycans. However, glycosylation did not directly contribute to the binding affinity between HCoV-19 S and hACE2. Additional post-translational modification (PTM) was identified, including multiple methylated sites in both proteins and multiple sites with hydrox... More

关键词

COVID-19, N-glycosylation, Spike protein, Structure, hACE2