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"CRISPR gene editing in yeast: An experimental protocol for an upper-division undergraduate laboratory course"

Biochem Mol Biol Educ. 2019; 
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GenParts™ DNA Fragments … from a single colony. The gap repair DNA fragments for ADE2 and STE12 (Supporting Information 3) were commercially synthesized and cloned into the EcoRV site of pUC57 by Genscript. These plasmids were also transformed … Get A Quote

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