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Molecular Typing, Antibiogram and PCR-RFLP Based Detection of Complex Isolated from

Pathogens. 2020; 
Abdelazeem M Algammal, Mohamed Fathi Mohamed, Basma A Tawfiek, Wael N Hozzein, Waleed M El Kazzaz, Mahmoud Mabrok
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Molecular Biology Tools … gene of those isolates was performed. Prior enzymes selections, the restriction cutting maps were analyzed using GenScript Restriction Enzyme Map Analysis Online Tools. Digestive enzyme acronym, sequence, site lengths … Get A Quote

摘要

Motile Aeromonas septicemia is a common bacterial disease that affects and causes tremendous economic losses globally. In order to investigate the prevalence, molecular typing, antibiogram and the biodiversity of complex, a total of 250 tilapia () were collected randomly from 10 private tilapia farms (25 fish/farm) at El-Sharkia Governorate, Egypt. The collected fish were subjected to clinical and bacteriological examinations. The majority of infected fish displayed ulcerative necrosis, exophthalmia, and internal signs of hemorrhagic septicemia. The prevalence of complex was 13.2%, where the liver was the most predominant affected organ (54.1%). Polymerase chain reaction (PCR) was used to verify the identifi... More

关键词

A. hydrophila complex, O. niloticus, PCR, RFLP, antibiogram