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Transient expression of the full-length glycoprotein from infectious hematopoietic necrosis virus in bean (Phaseolus vulgaris) leaves via agroinfiltration

Biotechnol Appl Biochem. 2020; 
Leila Fazeli, Pooran Golkar, Neda Mirakhorli, Seyed Amir Hossein Jalali, Rezvan Mohammadinezhad
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Recombinant Antibody Expression … Codon Adaptation Index (CAI) for expression in dicotyledon host (CAI of 0.68, Gene Script: https://www.genscript.com/codon-opt.html). The recombinant pPZP-IHN-G-His vector, as well as the non-recombinant pPZPY122 were introduced into A. tumefaciens strain GV3850 … Get A Quote

摘要

The glycoprotein of infectious hematopoietic necrosis virus (IHNV), the causative agent of acute disease in salmonids, is the only structural protein of the virus that can induce protective immunity in the fish host. Here, the reliability of bean (Phaseolus vulgaris) plant for the production of this viral protein was examined by the transient expression method. Using the syringe agroinfiltration method, leaves of bean plants were transformed with the expression construct encoding the full-length of IHNV glycoprotein (IHNV-G) gene. Furthermore, the transformation efficacy of two infiltration buffers including PBS-A (PBS+acetosyringone) and MMS-A (MES buffer + MgSO  + sucrose + acetosyringone) was compared.... More

关键词

agroinfiltration, glycoprotein, infectious hematopoietic necrosis virus, membrane protein, recombinant protein, transient transformation