Products/Services Used | Details | Operation |
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Recombinant Antibody Expression> | … per manufacturer's instructions. Recombinant VP4b (prepared as described in [19]) and recombinant Vago and Vir-1 proteins (Genscript, Piscataway, NJ, USA) were used as positive controls during analysis. Whole fly protein … | Get A Quote |
Study of the novel RNA virus, Nora virus, which is a persistent, picorna-like virus that replicates in the gut of offers insight into human innate immunity and other picorna-like viruses. Nora virus infection leads to a locomotor abnormality and upregulation of two candidate target proteins, Vago and Virus-induced RNA 1 (Vir-1). These proteins are uncharacterized in response to Nora virus. We hypothesize that Nora virus is circulating in the hemolymph of Nora virus-infected , allowing for migration beyond the primary site of replication in the gut. Analysis by qRT-PCR demonstrated biphasic viral load and corresponding and transcription levels, suggesting transcription of and occurs in response to viral inf... More