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The cell-penetrating YopM protein-functionalized quantum dot-plasmid DNA conjugate as a novel gene delivery vector

Plasmid. 2020; 
Özge Uğurlu, Fırat Barış Barlas, Serap Evran, Suna Timur
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Recombinant Antibody Expression … 2. Materials and methods. 2.1. Recombinant expression and purification of YopM. The codon optimized genes encoding YopM proteins from the three species of Yersinia were obtained from Genscript (Piscataway, NJ, USA) … Get A Quote

摘要

Non-viral gene delivery systems have great potential for safe and efficient gene therapy, while inefficient cellular and nuclear uptake remain as the major hurdles. Novel approaches are needed to enhance the transfection efficiency of non-viral vectors. In accordance with this need, the objective of this study was to construct a non-viral vector that could achieve gene delivery without using additional lipid-based transfection agent. We aimed to impart self-delivery property to a non-viral vector by using the cell and nucleus penetrating properties of YopM proteins from the three Yersinia spp. (Y. pestis, Y. enterocolotica and Y. pseudotuberculosis). Plasmid DNA (pDNA) encoding green fluorescent protein (GFP) w... More

关键词

Gene delivery, Non-viral vector, Quantum dots (QDs), YopM protein, pDNA