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Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis

Biosens Bioelectron. 2020; 
Taís Aparecida Reis Cordeiro, Helen Rodrigues Martins, Diego Leoni Franco, Fred Luciano Neves Santos, Paola Alejandra Fiorani Celedon, Vinícius Lopes Cantuária, Marta de Lana, Alexandre Barbosa Reis, Lucas Franco Ferreira
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Recombinant Antibody Expression … The T. cruzi-chimeric antigen IBMP 8.1 was obtained according to the methods reported by Santos et al. (2016a). Briefly, the T. cruzi recombinant gene was optimized for Escherichia coli expression and synthesized by a commercial supplier (GenScript, Piscataway, NJ, USA) … Get A Quote

摘要

In this work, a dual detection system based on an impedimetric immunosensor was developed for the first time for the simultaneous detection of anti-Trypanosoma cruzi and anti-Leishmania infantum antibodies in human and dog serum samples. The IBMP 8.1 and rLci1A/rLci2B recombinant antigens were immobilized over the surface of dual screen-printed carbon electrodes (W1 and W2) modified with poly (4-hydroxyphenylacetic acid). Under optimized conditions, the immunosensor recognized specific interactions for anti-T. cruzi antibodies up to a dilution of 1:10,240 and for anti-L. infantum up to 1:5120 in canine serum samples. Relative standard deviation (RSD) values of 2.8% for W1 and 3.6% for W2 were obtained for T. cr... More

关键词

Chagas, Dual detection, Electropolymerization, Impedimetric immunosensor, Visceral leishmaniasis