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Characterization of Calcium-Dependent Protein Kinases 3, a Protein Involved in Growth of

Front Microbiol. 2020; 
Qiang Zhang, Yaqiong Guo, Na Li, Yu Li, Jiayuan Su, Rui Xu, Ziding Zhang, Yaoyu Feng, Lihua Xiao
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Recombinant Antibody Expression … Polyclonal antibodies against recombinant CpCDPK3 were generated through immunizations of specific pathogen-free rabbits using Freund's complete and incomplete adjuvants by the GenScript Corporation (Nanjing, China) … Get A Quote

摘要

Calcium-dependent protein kinases (CDPKs) are considered promising targets for pharmaceutical intervention of cryptosporidiosis. Whole-genome sequencing has revealed the presence of several CDPKs (CDPKs) in . In this study, we expressed recombinant CDPK3 encoded by the gene in . The biologic characteristics and functions of CDPK3 were examined using qRT-PCR, immunofluorescence microscopy, and neutralization assay. The expression of the gene peaked in merozoites during culture while the CDPK3 protein was expressed in both sporozoites and merozoites. Polyclonal antibodies against CDPK3 showed no significant inhibitory effects on host invasion by the parasites. We assessed the inhibitory effects of 46 candidat... More

关键词

Cryptosporidium parvum, calcium-dependent protein kinase 3, enzyme, growth, inhibitor