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Dissecting the potential role of hepatitis E virus ORF1 nonstructural gene in cross-species infection by using intergenotypic chimeric viruses

J Med Virol. 2020; 
Debin Tian, Danielle M Yugo, Scott P Kenney, C Lynn Heffron, Tanja Opriessnig, Anbu K Karuppannan, Jenna Bayne, Patrick G Halbur, Xiang-Jin Meng
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GenParts™ DNA Fragments … genomic sequence, including the ORF1 that is derived from HEV-3 Meng strain clone pSHEV3, and the ORF2 and ORF3 that are derived from clone SKHEV2-MFA, was custom synthesized (GenScript, Piscataway, NJ). The genomic fragments coding for the HVR-X … Get A Quote

摘要

Hepatitis E virus (HEV) infects humans and more than a dozen other animal species. We previously showed that open reading frame 2 (ORF2) and ORF3 are apparently not involved in HEV cross-species infection, which infers that the ORF1 may contribute to host tropism. In this study, we utilize the genomic backbone of HEV-1 which only infects humans to construct a panel of intergenotypic chimeras in which the entire ORF1 gene or its functional domains were swapped with the corresponding regions from HEV-3 that infects both humans and pigs. We demonstrated that the chimeric HEVs were replication competent in human liver cells. Subsequently, we intrahepatically inoculated the RNA transcripts of chimeras into pigs to d... More

关键词

cross-species infection, hepatitis E virus (HEV), host tropism, intergenotypic chimeric viruses, pig, zoonotic infection