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Epithelial-Mesenchymal Transition in Asthma Airway Remodeling Is Regulated by the IL-33/CD146 Axis

Front Immunol. 2020; 
Zhixiao Sun, Ningfei Ji, Qiyun Ma, Ranran Zhu, Zhongqi Chen, Zhengxia Wang, Yan Qian, Chaojie Wu, Fan Hu, Mao Huang, Mingshun Zhang
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Proteins, Expression, Isolation and Analysis … To exclude the potential effects of lipopolysaccharide (LPS) contamination, HDM extract was treated with the ToxinEraser TM endotoxin removal Kit (L00338, Genscript, China). The purified product was the major constituent of HDM. Cell Transfection … Get A Quote

摘要

Epithelial-mesenchymal transition (EMT) is essential in asthma airway remodeling. IL-33 from epithelial cells is involved in pulmonary fibrosis. CD146 has been extensively explored in cancer-associated EMT. Whether IL-33 regulates CD146 in the EMT process associated with asthma airway remodeling is still largely unknown. We hypothesized that EMT in airway remodeling was regulated by the IL-33/CD146 axis. House dust mite (HDM) extract increased the expression of IL-33 and CD146 in epithelial cells. Increased expression of CD146 in HDM-treated epithelial cells could be blocked with an ST2-neutralizing antibody. Moreover, HDM-induced EMT was dependent on the CD146 and TGF-β/SMAD-3 signaling pathways. IL-33 defici... More

关键词

CD146, IL-33, allergy, asthma, epithelial-mesenchymal transition