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Bone Morphogenetic Protein-2 Conjugated to Quantum Dots is Biologically Functional

Nanomaterials (Basel). 2020; 
Daniel Halloran, Vrathasha Vrathasha, Hilary W Durbano, Anja Nohe
Products/Services Used Details Operation
Recombinant Antibody Expression … For 30 min in the dark, 10 μL of 40 nM recombinant BMP-2 (GenScript, Piscataway, NJ, USA) was placed in a solution with 2 μL of 8 μM QDot ® s (QDot ® 525 ITK carboxyl quantum dots, catalog #Q21341MP, Invitrogen, Carlsbad, CA, USA) and the proceeding reagents: 84 μL … Get A Quote

摘要

Quantum Dots (QDots) are novel, semi-conductive nanostructures that emit a certain fluorescence when excited by specific wavelengths. QDots are more photostable, brighter, and photobleach less than other fluorescent dyes. These characteristics give them the potential to be used in many biological applications. The shells of QDots are coated with functional groups, such as carboxylate and organic groups, allowing them to couple to peptides/proteins and be used for real-time imaging and high-resolution microscopy. Here, we utilize Quantum Dots and Bone Morphogenetic Protein-2 (BMP-2) to create a BMP-2-QDots conjugate. BMP-2 is a growth factor that drives many processes such as cardiogenesis, neural growth, and os... More

关键词

BMP-2, Bone: QDot®s, FTIR, fluorescence imaging, mineralization