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Insect Expression> | … GST-PSG1 was generated in Hi5 insect cells as previously reported (Snyder et al. 2001). The human Gal-1 cDNA was synthesized by GenScript USA Inc. (Piscataway, NJ) cloned into pFUSE-IgG1-e3-Fc1 and the protein was generated by transient transfection of Expi293 … | Get A Quote |
Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester. PSG1 has seven potential N-linked glycosylation sites across its four domains. We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites. Using different techniques, we explored whether PSG1 can bind to galectin-1 (Gal-1) as these two proteins were previously shown to participate in... More