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Synthetic Peptides Containing Three Neutralizing Epitopes of Genotype 4 Swine Hepatitis E Virus ORF2 induced Protection against Swine HEV Infection in Rabbit

Vaccines (Basel). 2020; 
Yiyang Chen, Tianxiang Chen, Yuhang Luo, Jie Fan, Meimei Zhang, Qin Zhao, Yuchen Nan, Baoyuan Liu, En-Min Zhou
Products/Services Used Details Operation
ORF cDNA Clones/MolecularCloud … serum albumin (BSA) for ELISA detection. All peptides used in this study were synthesized by GenScript (Nanjing, China) and were diluted according to the manufacturer's recommendations. Swine HEV truncated ORF2 protein … Get A Quote

摘要

Genotype 4 hepatitis E virus (HEV) is a zoonotic pathogen transmitted to humans through food and water. Previously, three genotype 4 swine HEV ORF2 peptides (EPTV, VKLYTS, and PSRPF) were identified as epitopes of virus-neutralizing monoclonal antibodies that partially blocked rabbit infection with swine HEV. Here, individual and tandem fused peptides were synthesized, conjugated to keyhole limpet hemocyanin (KLH), then evaluated for immunoprotection of rabbits against swine HEV infection. Forty New Zealand White rabbits were randomly assigned to eight groups; groups 1 thru 5 received three immunizations with EPTV-KLH, VKLYTS-KLH, PSRPF-KLH, EPTVKLYTS-KLH, or EPTVKLYTSPSRPF-KLH, respectively; group 6 received t... More

关键词

hepatitis E virus, neutralizing epitopes, swine HEV, synthetic peptides