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Nucleus-located PDK1 regulates growth, invasion and migration of breast cancer cells

Life Sci. 2020; 
Delu Gan, Shujun Yue, Yulin Jiang, Dian Zhang, He Shi, Husun Qian, Ting Zhou, Wenli Fang, Mengli Yao, Guowei Zuo, Tingmei Chen
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Mutagenesis Services … 2.5. Plasmids, mutagenesis, transfection. The pcDNA3.1(+) negative control plasmid, pcDNA3.1-Flag-PDK1-WT-GFP plasmid and pcDNA3.1-Flag-PDK1-MUT-GFP plasmid were purchased from GenScript (Wu Han, China) … Get A Quote

摘要

objective: It is well known that pyruvate dehydrogenase kinase 1 (PDK1) is highly expressed in breast cancer (BC) tissues and promotes tumor growth, but the underlying mechanisms of this process are unclear. Here, we investigated the effects of nuclear PDK1 on growth, migration and invasion in human BC cells. methods: The sub-cellular localization of PDK1 in BC cells was performed with subcellular fractionation followed by Western blot and immunofluorescence. The localization of PDK1 in breast normal tissue and breast duct carcinoma was detected by Immunohistochemistry. Then the protein-protein interaction between PDK1 and Importin β was verified by co-immunoprecipitation assay. Finally, the effects of nuclear... More

关键词

Apoptosis, Breast cancer, Invasion, Migration, Nuclear PDK1