| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | … A small hairpin RNA‐targeting ENOPH1 and a control sequence are shown in Table S1. The overexpression plasmid, pcDNA3.1‐C‐(k)DKY, encoded the ENOPH1 protein (cloning ID: X84811; GenScript, Nanjing, China). 2.5 Quantitative polymerase chain reaction (qPCR) … | Get A Quote |
Enolase-phosphatase 1 (ENOPH1), a newly identified enzyme involved in l-methionine biosynthesis, is associated with anxiety and depression. In this study, ENOPH1 was found to play a crucial role in promoting the proliferation and migration of glioma cells. Among high-grade glioma patients, the overall survival of the group showing high ENOPH1 expression was shorter than that of the group showing low ENOPH1 expression. ENOPH1 knockdown inhibited glioma cell proliferation and migration. In parallel, ENOPH1 knockdown suppressed tumor growth capacity and prolonged survival in an orthotopic glioma model. Mechanistically, we found that ENOPH1 activates the PI3K/AKT/mTOR signaling pathway by regulating THEM4. In concl... More
