Products/Services Used | Details | Operation |
---|---|---|
ORF cDNA Clones/MolecularCloud> | … PARP1 cDNA ORF clone (Cat #: OHu2551) and control vector (pcDNA3.1+/C- (K)DYK, Cat # SC1849) were purchased from GenScript. Transfection was carried out using Opti … gRNA from Genscript (gRNA2 and 3). Non-targeting gRNA lentivirus vector was from Genscript … | Get A Quote |
Nicotinamide adenine dinucleotide (NAD+) is an essential cofactor metabolite and is the currency of metabolic transactions critical for cell survival. Depending on tissue context and genotype, cancer cells have unique dependencies on NAD+ metabolic pathways. Poly(ADP-ribose) polymerases (PARPs) catalyze oligomerization of NAD+ monomers into poly(ADP-ribose) (PAR) chains during cellular response to alkylating chemotherapeutics, including procarbazine or temozolomide. Here, we find that, in endogenous IDH1 mutant tumor models, alkylator-induced cytotoxicity is markedly augmented by pharmacologic inhibition or genetic knockout of the PAR breakdown enzyme poly(ADP-ribose) glycohydrolase (PARG). Both in vitro and in... More