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Recombinant Antibody Expression> | … Recombinant Lcn-1 was produced as described previously (Holzfeind and Redl, 1994). A codon-optimized Fel d 4 gene was synthesized (GenScript, USA, Piscataway Township), Page 12. Heparan sulphate proteoglycan in lipocalin uptake 11 / 37 … | Get A Quote |
Lipocalins, small extracellular hydrophobic molecule carriers, can be internalized by a variety of different cells. However, to date receptors have only been identified for human lipocalins. Here, we specifically investigated uptake mechanisms for lipocalins ß-lactoglobulin and Fel d 4 in HeLa and Chinese hamster ovary (CHO) cells. We provide evidence that cell surface heparan sulphate proteoglycan is essential for internalization of these lipocalins. In HeLa cells, lipocalin uptake was inhibited by competition with soluble heparin, enzymatic digestion of cellular heparan sulphate by heparinase and inhibition of its biosynthesis by sodium chlorate. Biochemical studies by heparin affinity chromatography and col... More