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PCR Cloning and Subcloning> | … 2.2. Cloning Procedures and Plasmid Constructs. The open reading frame (ORF) of Mlp124357 without the portion coding for its signal peptide was obtained from GenScript (Piscataway, NJ, USA). The PDI-11 coding sequence was amplified from Arabidopsis … | Get A Quote |
, the causal agent of leaf rust, secretes an array of effectors into the host through the haustorium to gain nutrients and suppress immunity. The precise mechanisms by which these effectors promote virulence remain unclear. To address this question, we developed a transgenic line expressing a candidate effector, Mlp124357. Constitutive expression of the effector increased plant susceptibility to pathogens. A GxxxG motif present in Mlp124357 is required for its subcellular localization at the vacuolar membrane of the plant cell, as replacement of the glycine residues with alanines led to the delocalization of Mlp124357 to the nucleus and cytoplasm. We used immunoprecipitation and mass spectrometry (MS) to iden... More