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PCR Cloning and Subcloning> | … CTGTTCtccggagctGATGCTAGCCTGCCGTG GCGCAAGC) and SH-SY5Y cDNA as a template. All the subsequent steps involved Gibson cloning (CloneEZ PCR Cloning kit, GenScript). Myc tag was inserted into the pcDNA5-FRT … | Get A Quote |
Cold atmospheric plasma is an ionized gas that shows promise in regenerative medical treatments, yet the mechanisms underlying its effects are still poorly understood. Plasma treatment promotes cell growth or cell death depending on the cell type and exposure parameters. To date, no early cell response to plasma, such as stress granule (SG) formation has been addressed. Cytoplasmic SGs are formed as an immediate cell response to acute stress stimuli by recruitment of over 140 proteins intertwined with cytoplasmic RNAs that leads to transient suspension of protein translation. Encouraged by the plasma effects in regenerative medicine and oncology, the atmospheric pressure plasma jet with argon gas flow is being ... More