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Mutagenesis Services> | … Luciferase Reporter Assay Wild-type/mutant IGF1R luciferase reporter vector was synthesized by Genscript (Nanjing, Jiangsu, China), and U-CH1 cells were co-transfected with the wild-type /mutant IGF1R luciferase reporter vector and miR-100- 5p mimic/NC using … | Get A Quote |
unassigned: Our research aimed to illuminate the role of miR-100-5p in chordoma and potential mechanism. unassigned: We used microRNA array analysis to explore differentially expressed miRNAs in chordoma tissue and then verified by qRT-PCR. Cell proliferation and transwell assay were used to evaluate the function of miR-100-5p. Cell apoptosis was analyzed by flow cytometry, and using biological software, we predicted that the insulin-like growth factor 1 receptor (IGF1R) could be the target gene of miR-100-5p, which was then validated by dual luciferase assays and Western blot. unassigned: miR-100-5p was downregulated in chordoma tissues. Overexpression of miR-100-5p could suppress the growth of chordoma both i... More