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A Toxoplasma gondii patatin-like phospholipase contributes to host cell invasion

PLoS Pathog. 2020-07; 
Sarah K Wilson , Justine Heckendorn , Bruno Martorelli Di Genova , Lindsey L Koch , Peggy J Rooney , Naomi Morrissette , Maryse Lebrun , Laura J Knoll
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Peptoid Synthesis Three peptides were synthesized (Genscript) from unique locations along the gene based on maximizing the estimated immunogenicity and minimizing cross reactivity Get A Quote

摘要

Toxoplasma gondii is an obligate intracellular parasite that can invade any nucleated cell of any warm-blooded animal. In a previous screen to identify virulence determinants, disruption of gene TgME49_305140 generated a T. gondii mutant that could not establish a chronic infection in mice. The protein product of TgME49_305140, here named TgPL3, is a 277 kDa protein with a patatin-like phospholipase (PLP) domain and a microtubule binding domain. Antibodies generated against TgPL3 show that it is localized to the apical cap. Using a rapid selection FACS-based CRISPR/Cas-9 method, a TgPL3 deletion strain (ΔTgPL3) was generated. ΔTgPL3 parasites have defects in host cell invasion, which may be caused by reduced ... More

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