至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Organoarsenical tolerance in Sphingobacterium wenxiniae, a bacterium isolated from activated sludge

Environ Microbiol. 2021-05; 
Jian Chen, Jun Zhang, Barry P Rosen
Products/Services Used Details Operation
PCR Cloning and Subcloning The arsU1 gene was chemically synthesized with 5'-NdeI and 3-XhoI sites at each end with codon optimization for expression in E. coli and subcloned into plasmid pET29a(+) (GenScript, NJ, USA) plasmid pUC57-Kan (GenScript, NJ, USA) Get A Quote

摘要

Organoarsenicals enter the environment from biogenic and anthropogenic sources. Trivalent inorganic arsenite (As(III)) is microbially methylated to more toxic methylarsenite (MAs(III)) and dimethylarsenite (DMAs(III)) that oxidize in air to MAs(V) and DMAs(V). Sources include the herbicide monosodium methylarsenate (MSMA or MAs(V)), which is microbially reduced to MAs(III), and the aromatic arsenical roxarsone (3-nitro-4-hydroxybenzenearsonic acid or Rox), an antimicrobial growth promoter for poultry and swine. Here we show that Sphingobacterium wenxiniae LQY-18 , isolated from activated sludge, is resistant to trivalent MAs(III) and Rox(III). Sphingobacterium wenxiniae detoxifies MAs(III) and Rox(III) by oxida... More

关键词