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Sensitive and label-free chemiluminescence detection of malathion using exonuclease-assisted dual signal amplification and G-quadruplex/hemin DNAzyme

Journal of Hazardous Materials . 2020-12; 
Hao Wu, Jun Wu, Hongyong Wang, Yaling Liu, Guoqing Hana Pei Zou
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Single-Stranded DNA Synthesis The oligonucleotides used in this study (S1, S2, S3, and H1) were synthesized and HPLC-purified by Genscript (Nanjing) Co., Ltd. (Jiangsu, China). Get A Quote
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摘要

Malathion is one of the most commonly used organophosphorus pesticides that can cause serious harm to the ecological environment and human health. Herein, we demonstrated a label-free chemiluminescent aptasensor for the sensitive detection of malathion based on exonuclease-assisted dual signal amplification and G-quadruplex/hemin DNAzyme. Upon the addition of malathion, the aptamer probe specifically bound to the target to form a complex malathion-S3, leaving a duplex S1-S2. The complex malathion-S3 was digested by exonuclease I and the target was released. The released target was recycled to perform exonuclease I-assisted signal amplification. Furthermore, after treatment with exonuclease III, the duplex S1-S2... More

关键词

Malathion Aptasensor Dual signal amplification G-quadruplex DNAzyme Traditional Chinese medicine